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hammerhead hh ribozymes  (Addgene inc)


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    Addgene inc hammerhead hh ribozymes
    Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with <t>ribozymes</t> were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.
    Hammerhead Hh Ribozymes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hammerhead+hh+ribozymes/pYPQ141-ZmUbi-RZ-Lb+(Plasmid+%2386197)/pmc10894265-68-6-12
    Average 92 stars, based on 11 article reviews
    hammerhead hh ribozymes - by Bioz Stars, 2026-09
    92/100 stars

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    1) Product Images from "Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells"

    Article Title: Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-55088-4

    Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with ribozymes were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.
    Figure Legend Snippet: Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with ribozymes were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.

    Techniques Used: Expressing, Mutagenesis



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    Image Search Results


    Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with ribozymes were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.

    Journal: Scientific Reports

    Article Title: Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells

    doi: 10.1038/s41598-024-55088-4

    Figure Lengend Snippet: Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with ribozymes were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.

    Article Snippet: The Hepatitis Delta Virus (HDV) and Hammerhead (HH) ribozymes were amplified from Addgene plasmid #86197, which was a gift from Tang et al ., and similarly cloned to pGEM-T Easy (Promega).

    Techniques: Expressing, Mutagenesis